| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species
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| Autores: |
Wasko,Adriane P.
Severino,Fábio E.
Presti,Flávia T.
Poletto,Andréia B.
Martins,Cesar
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| Data: |
2007-01-01
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| Ano: |
2007
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| Palavras-chave: |
Actin gene
Amino acid residues
Gene structure
Nile tilapia
Nucleotide sequence
Oreochromis niloticus
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| Resumo: |
An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600010
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572007000600010
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.30 n.4 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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